Alport syndrome (AS) is a hereditary disease that leads to kidney failure and is caused by mutations in the COL4A3, COL4A4, and COL4A5 genes that lead to the absence of collagen α3α4α5 (IV) networks in the mature kidney glomerular basement membrane. Approximately 80% of AS is X-linked because of mutations in COL4A5, the gene encoding the alpha 5 chain of type IV collagen.
To investigate the pathogenesis of AS at the genetic level, we generated induced pluripotent stem cells (iPSCs) from renal tubular cells of a patient with AS. The successful iPSC generation laid the foundation to master the repair of the COL4A5 gene and to evaluate the differentiation of iPSC into Sertoli cells and the accompanying epigenetic changes at each stage. The generation of iPSCs from AS patients not only confirms that iPSCs could be generated from renal tubular cells , but also provides a novel type of genetic therapy for AS patients. In this study, we generated iPSCs from renal tubular cells via ectopic expression of four transcription factors (Oct4, Sox2, c-myc, and Klf4).
Gram Positive Bacteria Antibody
10-2778
Fitzgerald
1 mg
EUR 425
Description: Purified Gram Positive Bacteria Monoclonal
Gram Positive Bacteria Antibody
10-2779
Fitzgerald
1 mg
EUR 425
Description: Purified Gram Positive Bacteria Monoclonal
Gram Positive Bacteria Antibody
abx021739-1mg
Abbexa
1 mg
EUR 1220.4
Gram Positive Bacteria Antibody
abx021739-400l
Abbexa
400 µl
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Gram Positive Bacteria Antibody
abx021739-80l
Abbexa
80 µl
EUR 1150
Gram Positive Bacteria Antibody
abx021740-1mg
Abbexa
1 mg
EUR 1220.4
Gram Positive Bacteria Antibody
abx021740-400l
Abbexa
400 µl
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Gram Positive Bacteria Antibody
abx021740-80l
Abbexa
80 µl
EUR 1150
Gram Positive Bacteria Antibody
GWB-87FC21
GenWay Biotech
0.1 mg
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Gram positive bacteria, Antibody
GWB-CABB63
GenWay Biotech
0.1 mg Purified
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Gram Positive Bacteria Antibody
GWB-6D0DCC
GenWay Biotech
0.1 mg
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Gram Positive Bacteria Antibody
MBS5310938-1mg
MyBiosource
1mg
EUR 940
Gram Positive Bacteria Antibody
MBS5310938-5x1mg
MyBiosource
5x1mg
EUR 4070
Gram Positive Bacteria Antibody
MBS5310939-1mg
MyBiosource
1mg
EUR 940
Gram Positive Bacteria Antibody
MBS5310939-5x1mg
MyBiosource
5x1mg
EUR 4070
Gram Positive Bacteria Antibody
MBS5310940-1mg
MyBiosource
1mg
EUR 720
Gram Positive Bacteria Antibody
MBS5310940-5x1mg
MyBiosource
5x1mg
EUR 3095
Gram Positive Bacteria Antibody
MBS5310941-1mg
MyBiosource
1mg
EUR 720
Gram Positive Bacteria Antibody
MBS5310941-5x1mg
MyBiosource
5x1mg
EUR 3095
Anti-Gram Positive Bacteria Antibody
MBS4159311-01mg
MyBiosource
0.1mg
EUR 635
Anti-Gram Positive Bacteria Antibody
MBS4159311-5x01mg
MyBiosource
5x0.1mg
EUR 2610
Gram - bacteria
1054
Virostat
each
EUR 270
Description: LPS
Gram - bacteria
1055
Virostat
100 ug
EUR 270
Description: LPS
Gram + bacteria
3802
Virostat
100 ug
EUR 270
Description: LTA
Gram + bacteria
3803
Virostat
100 ug
EUR 270
Description: LTA
Gram + bacteria
3804
Virostat
each
EUR 270
Description: LTA
Gram + bacteria
3811
Virostat
100 ug
EUR 270
Description: LTA
Aerobic Bacteria
EPB-006
Creative BioMart
25 kg
EUR 2560
Description: Environmental Protection Microbiological Agents
Methane Bacteria
EPB-012
Creative BioMart
25 kg
EUR 2160
Description: Environmental Protection Microbiological Agents
Anaerobic Bacteria
EPB-007
Creative BioMart
25 kg
EUR 2160
Description: Environmental Protection Microbiological Agents
Nitrifying bacteria
EPB-001
Creative BioMart
25 kg
EUR 3200
Description: Environmental Protection Microbiological Agents
Denitrifying bacteria
EPB-002
Creative BioMart
25 kg
EUR 2560
Description: Environmental Protection Microbiological Agents
Methane Bacteria 2
EPB-015
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
Aerobic Bacteria 2
EPB-023
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
Deodorant bacteria 2
EPB-016
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
Bacteria Lysing Agent
EPB-030
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
COD Degrading Bacteria
EPB-008
Creative BioMart
25 kg
EUR 2160
Description: Environmental Protection Microbiological Agents
Nitrifying Bacteria 2
EPB-017
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
COD Degrading Bacteria
EPB-028
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
Gram positive bacteria
MBS320180-01mg
MyBiosource
0.1mg
EUR 355
Gram positive bacteria
MBS320180-5x01mg
MyBiosource
5x0.1mg
EUR 1500
Gram positive (+) bacteria
MBS320181-01mg
MyBiosource
0.1mg
EUR 355
Gram positive (+) bacteria
MBS320181-5x01mg
MyBiosource
5x0.1mg
EUR 1500
Gram positive bacteria
MBS328060-01mg
MyBiosource
0.1mg
EUR 355
Gram positive bacteria
MBS328060-5x01mg
MyBiosource
5x0.1mg
EUR 1500
Gram positive bacteria
MBS328061-01mg
MyBiosource
0.1mg
EUR 355
Gram positive bacteria
MBS328061-5x01mg
MyBiosource
5x0.1mg
EUR 1500
Denitrifying bacteria 2
EPB-018
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
Denitrifying bacteria 3
EPB-019
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
Sludge Digestion Bacteria
EPB-032
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
Complex nitrifying bacteria
EPB-003
Creative BioMart
25 kg
EUR 2560
Description: Environmental Protection Microbiological Agents
Complex biological bacteria
EPB-013
Creative BioMart
25 kg
EUR 2560
Description: Environmental Protection Microbiological Agents
Phosphorus Remover Bacteria
EPB-029
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
Bacteria Genomic DNA Kit
abx098080-100l
Abbexa
100 µl
EUR 300
Bacteria Genomic DNA Kit
abx098080-1ml
Abbexa
1 ml
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Bacteria Genomic DNA Kit
abx098080-200l
Abbexa
200 µl
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Bacteria Genomic DNA Kit
20-abx098080
Abbexa
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Bacteria RNA Plus Reagent
R402-01
Vazyme
20 ml
EUR 156
Gram Positive Bacteria mouse monoclonal antibody, clone BDI813, Purified
AM00856PU-N
Origene Technologies GmbH
1 mg
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Gram Positive Bacteria mouse monoclonal antibody, clone BDI380, Purified
AM00857PU-N
Origene Technologies GmbH
1 mg
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Bacteria for biological filter
EPB-011
Creative BioMart
25 kg
EUR 2960
Description: Environmental Protection Microbiological Agents
Complex biological bacteria 2
EPB-014
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
Anti-Gram-negative bacteria Endotoxin Monoclonal antibody, Clone 23-557.4
DMABT-Z60064
Creative Diagnostics
500 µg
EUR 945
Description: Mouse
Anti-Gram-negative bacteria Endotoxin Monoclonal antibody, Clone 23-334.6
DMABT-Z60333
Creative Diagnostics
500 µg
EUR 819
Description: Mouse
Acid Fast Bacteria Stain Kit
K1424-125
Biovision
each
EUR 405.6
Acid Fast Bacteria Stain Kit
K1424-30
Biovision
each
EUR 326.4
Necrotizing hepatopancreatitis bacteria
PCR-V281-PCRV28148D
Bioingentech
PCR-V281-48D
EUR 230
Necrotizing hepatopancreatitis bacteria
PCR-V281-PCRV28196D
Bioingentech
PCR-V281-96D
EUR 312
Necrotizing hepatopancreatitis bacteria
Oneq-V281-OneqV281100D
Bioingentech
Oneq-V281-100D
EUR 515
Necrotizing hepatopancreatitis bacteria
Oneq-V281-OneqV281150D
Bioingentech
Oneq-V281-150D
EUR 594
Necrotizing hepatopancreatitis bacteria
Oneq-V281-OneqV28150D
Bioingentech
Oneq-V281-50D
EUR 413
Lactic Bacteria Differential Agar
68888
Sisco Laboratories
100 Gms
EUR 9.41
Description: Part D
Lactic Bacteria Differential Agar
68888-1
Sisco Laboratories
500 Gms
EUR 35.92
Description: Part D
Lactic Bacteria Differential Agar
M1087-500G
EWC Diagnostics
1 unit
EUR 43.37
Description: Lactic Bacteria Differential Agar
Maltose Phosphorylase (MP (Bacteria))
MBS682032-5000Units
MyBiosource
5000Units
EUR 570
Maltose Phosphorylase (MP (Bacteria))
MBS682062-1000Units
MyBiosource
1000Units
EUR 350
Maltose Phosphorylase (MP (Bacteria))
MBS682122-250Units
MyBiosource
250Units
EUR 305
MAb to Gram Positive Bacteria
MBS310234-1mg
MyBiosource
1mg
EUR 915
MAb to Gram Positive Bacteria
MBS310234-5x1mg
MyBiosource
5x1mg
EUR 3945
MAb to Gram Positive Bacteria
MBS310317-1mg
MyBiosource
1mg
EUR 915
MAb to Gram Positive Bacteria
MBS310317-5x1mg
MyBiosource
5x1mg
EUR 3945
RNApure Bacteria Kit (DNase I)
MBS278037-50Preps
MyBiosource
50Preps
EUR 310
Psychrophilic Bacteria Count Plate
KGR029
Ring Biotechnology Co
12tests
EUR 20
Description: Enrichment, 21±1℃, 28-30h
Lactic Bacteria Differential Broth
M1086-500G
EWC Diagnostics
1 unit
EUR 41.3
Description: Lactic Bacteria Differential Broth
Lipopolysaccharide (LPS gram negative bacteria) goat polyclonal antibody, Purified
BP2235
Origene Technologies GmbH
1 ml
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Mbead Bacteria Genomic DNA Kit
PDM03-0100
Bio-Helix
100 rxns
EUR 154
Lactic Acid Bacteria Count Plate
KGR014
Ring Biotechnology Co
12tests
EUR 24
Description: Enrichment, 36℃±1℃,48±2h
Isolation Medium For Iron Bacteria
M622-100G
EWC Diagnostics
1 unit
EUR 14.77
Description: Isolation Medium For Iron Bacteria
Isolation Medium For Iron Bacteria
M622-500G
EWC Diagnostics
1 unit
EUR 43.27
Description: Isolation Medium For Iron Bacteria
Bacteria-Derived Collagenase Purified
MBS136183-4000Units
MyBiosource
4000Units
EUR 260
Bacteria-Derived Collagenase Purified
MBS136183-5x4000Units
MyBiosource
5x4000Units
EUR 985
Bacteria-Derived Collagenase Purified
IBCTCLSPF4KU
Innovative research
each
EUR 157
Description: Bacteria-Derived Collagenase Purified
Lysis Buffer for Bacteria, pH 8.0
22040068-1
Bio-WORLD
500 mL
EUR 60.27
Lysis Buffer for Bacteria, pH 8.0
22040068-2
Bio-WORLD
1 L
EUR 91.94
*Lactic Acid Bacteria Selective Agar
M1072-100G
EWC Diagnostics
1 unit
EUR 25.78
Description: *Lactic Acid Bacteria Selective Agar
*Lactic Acid Bacteria Selective Agar
M1072-500G
EWC Diagnostics
1 unit
EUR 103.82
Description: *Lactic Acid Bacteria Selective Agar
Bacteria-Derived Deoxyribonucleic Acid
MBS136185-10mg
MyBiosource
10mg
EUR 310
Bacteria-Derived Deoxyribonucleic Acid
MBS136185-5x10mg
MyBiosource
5x10mg
EUR 1210
Bacteria-Derived Deoxyribonucleic Acid
IBCTDNA10MG
Innovative research
each
EUR 199
Description: Bacteria-Derived Deoxyribonucleic Acid
GenUP Bacteria gDNA Kit (10 preps)
BRBR0700701
Westburg
each
EUR 73.58
GenUP Bacteria gDNA Kit (50 preps)
BRBR0700702
Westburg
each
EUR 239.8
GenUP Bacteria gDNA Kit (250 preps)
BRBR0700703
Westburg
each
EUR 981
Bacteria-Derived Diaphorase Lyophilized
MBS136186-1000Units
MyBiosource
1000Units
EUR 205
Bacteria-Derived Diaphorase Lyophilized
MBS136186-2000Units
MyBiosource
2000Units
EUR 255
Bacteria-Derived Diaphorase Lyophilized
MBS136186-5x2000Units
MyBiosource
5x2000Units
EUR 960
Bacteria-Derived Diaphorase Lyophilized
IBCTDTLY1KU
Innovative research
each
EUR 96
Description: Bacteria-Derived Diaphorase Lyophilized
Bacteria-Derived Diaphorase Lyophilized
IBCTDTLY2KU
Innovative research
each
EUR 143
Description: Bacteria-Derived Diaphorase Lyophilized
Bead Beating Tube Type A (Bacteria)
BB0108(C1BTA050)
Geneaid Biotech
each
EUR 50
human anti-Gram-negative bacteria LPS IgG
E4A11A65-G
EnoGene
100ug
EUR 995
human anti-Gram-negative bacteria LPS IgM
E4A11A65-M
EnoGene
100ug
EUR 995
Lactic Bacteria Differential HiVeg Agar
MV1087-500G
EWC Diagnostics
1 unit
EUR 43.37
Description: Lactic Bacteria Differential HiVeg Agar
genesig Easy kit for Bacteria Domain
Z-Path-Eubacteria-EASY
Novacyt Group
50 tests
EUR 410
Description: Eubacteria
Bacteria beta Galactosidase (GLB1) Protein
abx670038-5mg
Abbexa
5 mg
EUR 526.8
Chemical Sewage Degrading Bacteria Agent
EPB-026
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
Lactic Bacteria Differential HiVeg Broth
MV1086-500G
EWC Diagnostics
1 unit
EUR 41.3
Description: Lactic Bacteria Differential HiVeg Broth
Bacteria-Derived JM109 Competent Cells
MBS8430457-INQUIRE
MyBiosource
INQUIRE
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Anti gram-positive/negative bacteria agent 1
HY-132915
MedChemExpress
Get quote
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Description: Anti gram-positive/negative bacteria agent 1 is an antibiotic conjugate with an artificial MECAM-based siderophore.
Ammonia Nitrogen Degrading Bacteria Agent
EPB-027
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
Soil Remediation and Improvement Bacteria
EPB-031
Creative BioMart
25 kg
EUR 2000
Description: Environmental Protection Microbiological Agents
Gram Positive Bacteria DNA(Spin Column)
2550A
Unibiotest
50 Tests
EUR 300
Gram Positive Bacteria DNA(Spin Column)
2550B
Unibiotest
2*100 Tests
EUR 1200
innuPREP Bacteria DNA Kit - 10 reactions
IN845-KS-6010010
Westburg
each
EUR 44.15
innuPREP Bacteria DNA Kit - 50 reactions
IN845-KS-6010050
Westburg
each
EUR 168.41
innuPREP Bacteria DNA Kit - 250 reactions
IN845-KS-6010250
Westburg
each
EUR 495.95
Ribo-off rRNA Depletion Kit (Bacteria)
N407-01
Vazyme
12 rxn
EUR 507.6
Ribo-off rRNA Depletion Kit (Bacteria)
N407-02
Vazyme
24 rxn
EUR 816
Bacteria Genomic DNA Kit (No RNase A)
abx098240-100l
Abbexa
100 µl
EUR 287.5
Bacteria Genomic DNA Kit (No RNase A)
abx098240-1ml
Abbexa
1 ml
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Bacteria Genomic DNA Kit (No RNase A)
abx098240-200l
Abbexa
200 µl
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Bacteria Genomic DNA Kit (No RNase A)
abx098240-50rxns
Abbexa
50 rxns
EUR 276
Bacteria-Derived ATG3 Recombinant Protein
IBCTATG3R10UG
Innovative research
each
EUR 212
Description: Bacteria-Derived ATG3 Recombinant Protein
Bacteria-Derived ATG3 Recombinant Protein
MBS8430525-INQUIRE
MyBiosource
INQUIRE
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Urease from Bacteria, Recombinant: 141U/mg;
NATE-1035
Creative Enzymes
1 KU
EUR 1398
Description: > 150 U/mg
Bacteria-Derived GSTP1 Recombinant Protein
IBCTGSTP1R10UG
Innovative research
each
EUR 372
Description: Bacteria-Derived GSTP1 Recombinant Protein
Bacteria-Derived GSTP1 Recombinant Protein
MBS8430576-INQUIRE
MyBiosource
INQUIRE
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FastPure Bacteria DNA Isolation Mini Kit
DC103
Vazyme
100 rxn
EUR 212.4
FastPure Bacteria DNA Isolation Mini Kit
DC103-01
Vazyme
100 rxns
EUR 52
FastPure Bacteria DNA Isolation Mini Kit
DC103-01-100rxns
Vazyme
100 rxns
EUR 67.58
Bacteria RNA Purification Kit(Spin Column)
2119A
Unibiotest
20 Tests
EUR 100
Bacteria RNA Purification Kit(Spin Column)
2119B
Unibiotest
50 Tests
EUR 200
Bacteria RNA Purification Kit(Spin Column)
2119C
Unibiotest
4*50 Tests
EUR 600
Bacteria RNA Purification Kit(Spin Column)
2120A
Unibiotest
20 Tests
EUR 150
Bacteria RNA Purification Kit(Spin Column)
2120B
Unibiotest
50 Tests
EUR 250
Bacteria RNA Purification Kit(Spin Column)
2120C
Unibiotest
4*50 Tests
EUR 800
Bacteria-Derived Galactosidase Beta Purified
MBS136189-1000Units
MyBiosource
1000Units
EUR 230
Bacteria-Derived Galactosidase Beta Purified
MBS136189-5000Units
MyBiosource
5000Units
EUR 635
Bacteria-Derived Galactosidase Beta Purified
MBS136189-5x5000Units
MyBiosource
5x5000Units
EUR 2665
Bacteria-Derived Galactosidase Beta Purified
IBCTGLBB1KU
Innovative research
each
EUR 121
Description: Bacteria-Derived Galactosidase Beta Purified
Bacteria-Derived Galactosidase Beta Purified
IBCTGLBB5KU
Innovative research
each
EUR 493
Description: Bacteria-Derived Galactosidase Beta Purified
Adiponectin: Mouse (Recombinant protein; bacteria)
MBS398080-01mg
MyBiosource
0.1mg
EUR 345
According to the human embryonic stem cell (hESC) charter , iPSC formation was confirmed by comparatively analyzing hESC markers via colony morphology, immunohistochemistry, qRT-PCR, flow cytometry, gene expression profiling of the three germ layers, and karyotyping. Our results demonstrated that iPSCs were similar to hESCs with regard to morphology, proliferation, hESC-specific surface marker expression, and differentiation into the cell types of the three germ layers.
The efficient generation of iPSCs from the renal tubular cells of an AS patient would provide a novel model to investigate the mechanisms underlying AS and to develop new treatments for AS.
Generation of induced pluripotent stem cells from renal tubular cells of a patient with Alport syndrome.
The regulation of science and the Charter of Rights: would a ban on non-reproductive human cloning unjustifiably violate freedom of expression?
Non-Reproductive Human Cloning (NRHC) allows researchers to develop and clone cells , including non-reproductive cells , and to research the etiology and transmission of disease. The ability to clone specific stem cells may also allow researchers to clone cells with genetic defects and analyze those cells with more precisions. Despite those potential benefits, Parliament has banned such cloning due to a myriad of social and ethical concerns. In May 2002, the Canadian Government introduced Bill C-13 on assisted human reproductive technologies. Bill C-13 deals with both the scientific and the clinical use of human reproductive materials, and it prohibits a number of other activities, including NRHC. Although the Supreme Court of Canada has never ruled on whether scientific experiments area form of expression, academic support exists for this notion. The authors go through the legal analysis that would be required to find that scientific experiments are expression, focusing in part on whether NRHC could be considered violent and thus fall outside the protection of section 2(b). The latter question is complicated by the ongoing policy debate over whether an “embryonic cell ” is property of human life. The authors then consider whether a ban on NRHC could be justified under section 1 of the Charter . They conclude that both the breadth of the legislative purpose and the proportionality of the measure are problematic. Proportionality is a specific concern because the ban could be viewed as an outright denial of scientific freedom of expression. Although consistent with current jurisprudence on freedom of expression, this paper runs against the flow of government policy in the areas of regulation and prohibition of non-reproductive human cloning. As there has been no Charter litigation to date on whether scientific research is a form of expression, the authors introduce a new way of looking at the legality of the regulation of new reproductive technologies.