Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach.

The directed differentiation of pluripotent stem cells to a desired lineage often involves complex and lengthy protocols. In order to study the requirements for differentiation in a systematic way, we present here methodology for an iterative approach using combinations of small molecules and biological factors. The factors are used in a cyclical process in which the best combination of factors and concentrations is selected in one round of testing, followed by a modification of the combination and subsequent rounds. While this may produce the desired differentiation in the cell population under study, it is also possible that other strategies may be needed to optimize the differentiation process. These strategies are described in this chapter.

Making NSC and Neurons from Patient-Derived Tissue Samples.

The human brain and mechanisms underlying its functioning has been a field of intense research due to its complexity, inaccessibility, and the large numbers of debilitating disorders affecting this organ. Model organisms have provided great insight into the functioning of the mammalian brain; however, there exist many features unique to humans which need detailed understanding.

MeCP2 (Methyl-CpG Binding Protein 2), affinity purified antibody, rabbit
MBS555740-5x01mL	MyBiosource	5x0.1mL	EUR 1815

Affinity Purified Rabbit anti-Calmodulin Binding Protein Tag
MBS560452-1mg	MyBiosource	1mg	EUR 905

Affinity Purified Rabbit anti-Calmodulin Binding Protein Tag
MBS560452-5x1mg	MyBiosource	5x1mg	EUR 3920

Affinity Purified Rabbit anti-Calmodulin Binding Protein Tag
MBS560453-01mg	MyBiosource	0.1mg	EUR 290

Affinity Purified Rabbit anti-Calmodulin Binding Protein Tag
MBS560453-5x01mg	MyBiosource	5x0.1mg	EUR 1155

Affinity™ ECL kit (picogram)
KF001-100ul	Affinity Biosciences	100ul	EUR 60
Description: WB

Affinity™ ECL kit (picogram)
KF001-200ul	Affinity Biosciences	200ul	EUR 168
Description: WB

Affinity™ ECL kit (picogram)
KF8001	Affinity Biosciences	100ml	EUR 100
Description: N/A

Affinity™ ECL kit (picogram)
KF8001-100ml	Affinity Biosciences	100ml	EUR 60
Description: WB

Affinity™ ECL kit (picogram)
KF8001-500ml	Affinity Biosciences	500ml	EUR 168
Description: WB

Affinity™ ECL kit (femtogram)
KF003-100ul	Affinity Biosciences	100ul	EUR 120
Description: WB

Affinity™ ECL kit (femtogram)
KF003-200ul	Affinity Biosciences	200ul	EUR 300
Description: WB

Affinity™ ECL kit (femtogram)
KF8003	Affinity Biosciences	100ml	EUR 200
Description: N/A

Affinity™ ECL kit (femtogram)
KF8003-100ml	Affinity Biosciences	100ml	EUR 120
Description: WB

Affinity™ ECL kit (femtogram)
KF8003-500ml	Affinity Biosciences	500ml	EUR 300
Description: WB

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified
IRBACBPTAP100UG	Innovative research	each	EUR 404
Description: Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified
IRBACBPTAP1MG	Innovative research	each	EUR 1550
Description: Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified
MBS8421126-01mg	MyBiosource	0.1mg	EUR 555

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified
MBS8421126-1mg	MyBiosource	1mg	EUR 1890

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified
MBS8421126-5x1mg	MyBiosource	5x1mg	EUR 8310

Retinol Binding Protein (RBP), Polyclonal Anti-Human Affinity Purified Host Goat
MBS171050-10mg	MyBiosource	10mg	EUR 1710

Retinol Binding Protein (RBP), Polyclonal Anti-Human Affinity Purified Host Goat
MBS171050-1mg	MyBiosource	1mg	EUR 350

Retinol Binding Protein (RBP), Polyclonal Anti-Human Affinity Purified Host Goat
MBS171050-5x10mg	MyBiosource	5x10mg	EUR 7495

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified HRP Labeled
IRBACBPTAPHRP100UG	Innovative research	each	EUR 450
Description: Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified HRP Labeled

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified HRP Labeled
IRBACBPTAPHRP1MG	Innovative research	each	EUR 1598
Description: Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified HRP Labeled

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified HRP Labeled
MBS8421128-01mg	MyBiosource	0.1mg	EUR 610

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified HRP Labeled
MBS8421128-1mg	MyBiosource	1mg	EUR 1945

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified HRP Labeled
MBS8421128-5x1mg	MyBiosource	5x1mg	EUR 8560

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified FITC Labeled
IRBACBPTAPFITC100UG	Innovative research	each	EUR 425
Description: Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified FITC Labeled

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified FITC Labeled
IRBACBPTAPFITC1MG	Innovative research	each	EUR 1571
Description: Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified FITC Labeled

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified FITC Labeled
MBS8421127-01mg	MyBiosource	0.1mg	EUR 580

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified FITC Labeled
MBS8421127-1mg	MyBiosource	1mg	EUR 1915

Rabbit Anti Calmodulin Binding Peptide Tag Polyclonal Affinity Purified FITC Labeled
MBS8421127-5x1mg	MyBiosource	5x1mg	EUR 8420

D Box Binding Protein Antibody
DF13971-100ul	Affinity Biosciences	100ul	EUR 168
Description: IHC,ELISA(peptide)

D Box Binding Protein Antibody
DF13971-200ul	Affinity Biosciences	200ul	EUR 210
Description: IHC,ELISA(peptide)

Z DNA binding protein Antibody
DF14090-100ul	Affinity Biosciences	100ul	EUR 168
Description: IHC,IF/ICC,ELISA(peptide)

Z DNA binding protein Antibody
DF14090-200ul	Affinity Biosciences	200ul	EUR 210
Description: IHC,IF/ICC,ELISA(peptide)

Vitamin D Binding protein Antibody
DF7840-100ul	Affinity Biosciences	100ul	EUR 168
Description: WB,IF/ICC,ELISA(peptide)

Vitamin D Binding protein Antibody
DF7840-200ul	Affinity Biosciences	200ul	EUR 210
Description: WB,IF/ICC,ELISA(peptide)

AC Antibody affinity resin
HY-157235	MedChemExpress	Get quote	Ask for price
Description: AC Antibody affinity resin is AC commonly used packing. AC Antibody affinity resin has highly cross-linked agarose matrix with 85 μm of average particle size. AC Antibody affinity resin has alkali-resistant protein A derivative (E. coli) ligand. AC Antibody affinity resin can be used for antibody affinity chromatography[1].

HA Affinity Purified Antibody
MBS194421-1mg	MyBiosource	1mg	EUR 1110

HA Affinity Purified Antibody
MBS194422-INQUIRE	MyBiosource	INQUIRE	Ask for price

T7 Affinity Purified Antibody
MBS194425-INQUIRE	MyBiosource	INQUIRE	Ask for price

T7 Affinity Purified Antibody
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V5 Affinity Purified Antibody
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V5 Affinity Purified Antibody
MBS194428-INQUIRE	MyBiosource	INQUIRE	Ask for price

HA Affinity Purified Antibody
MBS194468-01mg	MyBiosource	0.1mg	EUR 480

HA Affinity Purified Antibody
MBS194468-5x01mg	MyBiosource	5x0.1mg	EUR 2140

T7 Affinity Purified Antibody
MBS194471-01mg	MyBiosource	0.1mg	EUR 480

T7 Affinity Purified Antibody
MBS194471-5x01mg	MyBiosource	5x0.1mg	EUR 2140

T7 Affinity Purified Antibody
MBS194472-01mg	MyBiosource	0.1mg	EUR 480

T7 Affinity Purified Antibody
MBS194472-5x01mg	MyBiosource	5x0.1mg	EUR 2140

V5 Affinity Purified Antibody
MBS194473-01mg	MyBiosource	0.1mg	EUR 480

V5 Affinity Purified Antibody
MBS194473-5x01mg	MyBiosource	5x0.1mg	EUR 2140

V5 Affinity Purified Antibody
MBS194474-01mg	MyBiosource	0.1mg	EUR 470

V5 Affinity Purified Antibody
MBS194474-5x01mg	MyBiosource	5x0.1mg	EUR 2110

D6 Antibody Affinity Purified
MBS540450-01mg	MyBiosource	0.1mg	EUR 445

D6 Antibody Affinity Purified
MBS540450-5x01mg	MyBiosource	5x0.1mg	EUR 1855

D6 Antibody Affinity Purified
MBS540586-01mg	MyBiosource	0.1mg	EUR 445

D6 Antibody Affinity Purified
MBS540586-5x01mg	MyBiosource	5x0.1mg	EUR 1855

AU1 Affinity Purified Antibody
MBS194409-1mg	MyBiosource	1mg	EUR 1160

AU1 Affinity Purified Antibody
MBS194410-INQUIRE	MyBiosource	INQUIRE	Ask for price

AU5 Affinity Purified Antibody
MBS194411-1mg	MyBiosource	1mg	EUR 1160

AU5 Affinity Purified Antibody
MBS194412-1mg	MyBiosource	1mg	EUR 1160

ECS Affinity Purified Antibody
MBS194415-INQUIRE	MyBiosource	INQUIRE	Ask for price

ECS Affinity Purified Antibody
MBS194416-1mg	MyBiosource	1mg	EUR 1160

KT3 Affinity Purified Antibody
MBS194423-1mg	MyBiosource	1mg	EUR 1160

KT3 Affinity Purified Antibody
MBS194424-1mg	MyBiosource	1mg	EUR 1160

AU1 Affinity Purified Antibody
MBS194455-01mg	MyBiosource	0.1mg	EUR 480

AU1 Affinity Purified Antibody
MBS194455-5x01mg	MyBiosource	5x0.1mg	EUR 2140

AU1 Affinity Purified Antibody
MBS194456-01mg	MyBiosource	0.1mg	EUR 470

AU1 Affinity Purified Antibody
MBS194456-5x01mg	MyBiosource	5x0.1mg	EUR 2110

AU5 Affinity Purified Antibody
MBS194457-01mg	MyBiosource	0.1mg	EUR 470

AU5 Affinity Purified Antibody
MBS194457-5x01mg	MyBiosource	5x0.1mg	EUR 2110

AU5 Affinity Purified Antibody
MBS194458-01mg	MyBiosource	0.1mg	EUR 480

AU5 Affinity Purified Antibody
MBS194458-5x01mg	MyBiosource	5x0.1mg	EUR 2140

ECS Affinity Purified Antibody
MBS194461-01mg	MyBiosource	0.1mg	EUR 480

ECS Affinity Purified Antibody
MBS194461-5x01mg	MyBiosource	5x0.1mg	EUR 2140

ECS Affinity Purified Antibody
MBS194462-01mg	MyBiosource	0.1mg	EUR 480

ECS Affinity Purified Antibody
MBS194462-5x01mg	MyBiosource	5x0.1mg	EUR 2140

KT3 Affinity Purified Antibody
MBS194469-01mg	MyBiosource	0.1mg	EUR 470

KT3 Affinity Purified Antibody
MBS194469-5x01mg	MyBiosource	5x0.1mg	EUR 2110

KT3 Affinity Purified Antibody
MBS194470-01mg	MyBiosource	0.1mg	EUR 470

KT3 Affinity Purified Antibody
MBS194470-5x01mg	MyBiosource	5x0.1mg	EUR 2110

trp Antibody Affinity Purified
MBS5400174-01mg	MyBiosource	0.1mg	EUR 445

trp Antibody Affinity Purified
MBS5400174-5x01mg	MyBiosource	5x0.1mg	EUR 1855

ILK Antibody Affinity Purified
MBS540038-01mg	MyBiosource	0.1mg	EUR 445

ILK Antibody Affinity Purified
MBS540038-5x01mg	MyBiosource	5x0.1mg	EUR 1855

LAT Antibody Affinity Purified
MBS541979-01mg	MyBiosource	0.1mg	EUR 445

LAT Antibody Affinity Purified
MBS541979-5x01mg	MyBiosource	5x0.1mg	EUR 1855

HE4 Antibody Affinity Purified
MBS540186-01mg	MyBiosource	0.1mg	EUR 445

HE4 Antibody Affinity Purified
MBS540186-5x01mg	MyBiosource	5x0.1mg	EUR 1855

ITK Antibody Affinity Purified
MBS540210-01mg	MyBiosource	0.1mg	EUR 445

ITK Antibody Affinity Purified
MBS540210-5x01mg	MyBiosource	5x0.1mg	EUR 1855

MSF Antibody Affinity Purified
MBS540397-01mg	MyBiosource	0.1mg	EUR 445

MSF Antibody Affinity Purified
MBS540397-5x01mg	MyBiosource	5x0.1mg	EUR 1855

FSH Antibody Affinity Purified
MBS540541-01mg	MyBiosource	0.1mg	EUR 445

FSH Antibody Affinity Purified
MBS540541-5x01mg	MyBiosource	5x0.1mg	EUR 1855

DCC Antibody Affinity Purified
MBS540561-01mg	MyBiosource	0.1mg	EUR 445

DCC Antibody Affinity Purified
MBS540561-5x01mg	MyBiosource	5x0.1mg	EUR 1855

CMT Antibody Affinity Purified
MBS540771-01mg	MyBiosource	0.1mg	EUR 445

CMT Antibody Affinity Purified
MBS540771-5x01mg	MyBiosource	5x0.1mg	EUR 1855

p63 Antibody Affinity Purified
MBS540812-01mg	MyBiosource	0.1mg	EUR 445

p63 Antibody Affinity Purified
MBS540812-5x01mg	MyBiosource	5x0.1mg	EUR 1855

FYN Antibody Affinity Purified
MBS540813-01mg	MyBiosource	0.1mg	EUR 445

FYN Antibody Affinity Purified
MBS540813-5x01mg	MyBiosource	5x0.1mg	EUR 1855

FGR Antibody Affinity Purified
MBS540827-01mg	MyBiosource	0.1mg	EUR 445

FGR Antibody Affinity Purified
MBS540827-5x01mg	MyBiosource	5x0.1mg	EUR 1855

EGR2, Affinity Purified Antibody
MBS555153-01mL	MyBiosource	0.1mL	EUR 435

EGR2, Affinity Purified Antibody
MBS555153-5x01mL	MyBiosource	5x0.1mL	EUR 1815

LYK5 Antibody Affinity Purified
MBS5400004-01mg	MyBiosource	0.1mg	EUR 445

LYK5 Antibody Affinity Purified
MBS5400004-5x01mg	MyBiosource	5x0.1mg	EUR 1855

TIA1 Antibody Affinity Purified
MBS5400062-01mg	MyBiosource	0.1mg	EUR 445

TIA1 Antibody Affinity Purified
MBS5400062-5x01mg	MyBiosource	5x0.1mg	EUR 1855

TIA1 Antibody Affinity Purified
MBS5400063-01mg	MyBiosource	0.1mg	EUR 445

TIA1 Antibody Affinity Purified
MBS5400063-5x01mg	MyBiosource	5x0.1mg	EUR 1855

TRF2 Antibody Affinity Purified
MBS5400165-01mg	MyBiosource	0.1mg	EUR 445

TRF2 Antibody Affinity Purified
MBS5400165-5x01mg	MyBiosource	5x0.1mg	EUR 1855

TRF2 Antibody Affinity Purified
MBS5400166-01mg	MyBiosource	0.1mg	EUR 445

TRF2 Antibody Affinity Purified
MBS5400166-5x01mg	MyBiosource	5x0.1mg	EUR 1855

XTP4 Antibody Affinity Purified
MBS5400275-01mg	MyBiosource	0.1mg	EUR 445

XTP4 Antibody Affinity Purified
MBS5400275-5x01mg	MyBiosource	5x0.1mg	EUR 1855

MIG7 Antibody Affinity Purified
MBS540040-01mg	MyBiosource	0.1mg	EUR 445

MIG7 Antibody Affinity Purified
MBS540040-5x01mg	MyBiosource	5x0.1mg	EUR 1855

P2Y1 Antibody Affinity Purified
MBS540047-01mg	MyBiosource	0.1mg	EUR 445

P2Y1 Antibody Affinity Purified
MBS540047-5x01mg	MyBiosource	5x0.1mg	EUR 1855

Nkx2.2 Antibody Affinity Purified
MBS542223-01mg	MyBiosource	0.1mg	EUR 445

Nkx2.2 Antibody Affinity Purified
MBS542223-5x01mg	MyBiosource	5x0.1mg	EUR 1855

OSR2 Antibody Affinity Purified
MBS542271-01mg	MyBiosource	0.1mg	EUR 445

OSR2 Antibody Affinity Purified
MBS542271-5x01mg	MyBiosource	5x0.1mg	EUR 1855

PELO Antibody Affinity Purified
MBS543624-01mg	MyBiosource	0.1mg	EUR 445

PELO Antibody Affinity Purified
MBS543624-5x01mg	MyBiosource	5x0.1mg	EUR 1855

PMS2 Antibody Affinity Purified
MBS544005-01mg	MyBiosource	0.1mg	EUR 445

PMS2 Antibody Affinity Purified
MBS544005-5x01mg	MyBiosource	5x0.1mg	EUR 1855

POP4 Antibody Affinity Purified
MBS544083-01mg	MyBiosource	0.1mg	EUR 445

POP4 Antibody Affinity Purified
MBS544083-5x01mg	MyBiosource	5x0.1mg	EUR 1855

POP4 Antibody Affinity Purified
MBS544084-01mg	MyBiosource	0.1mg	EUR 445

POP4 Antibody Affinity Purified
MBS544084-5x01mg	MyBiosource	5x0.1mg	EUR 1855

PGCF Antibody Affinity Purified
MBS540094-01mg	MyBiosource	0.1mg	EUR 490

PGCF Antibody Affinity Purified
MBS540094-5x01mg	MyBiosource	5x0.1mg	EUR 2065

SARA Antibody Affinity Purified
MBS540113-01mg	MyBiosource	0.1mg	EUR 445

SARA Antibody Affinity Purified
MBS540113-5x01mg	MyBiosource	5x0.1mg	EUR 1855

TRIF Antibody Affinity Purified
MBS540129-01mg	MyBiosource	0.1mg	EUR 445

TRIF Antibody Affinity Purified
MBS540129-5x01mg	MyBiosource	5x0.1mg	EUR 1855

ARTS Antibody Affinity Purified
MBS540165-01mg	MyBiosource	0.1mg	EUR 445

ARTS Antibody Affinity Purified
MBS540165-5x01mg	MyBiosource	5x0.1mg	EUR 1855

TLR7 Antibody Affinity Purified
MBS540166-01mg	MyBiosource	0.1mg	EUR 445

TLR7 Antibody Affinity Purified
MBS540166-5x01mg	MyBiosource	5x0.1mg	EUR 1855

UCN2 Antibody Affinity Purified
MBS540178-01mg	MyBiosource	0.1mg	EUR 445

UCN2 Antibody Affinity Purified
MBS540178-5x01mg	MyBiosource	5x0.1mg	EUR 1855

SARA Antibody Affinity Purified
MBS540231-01mg	MyBiosource	0.1mg	EUR 445

In this context, human pluripotent stem cells (HPSCs) have emerged as a promising resource.In the developing brain, cortical diversification is achieved by neural stem cells/neural progenitor cells (NSCs/NPCs) by altering its potency (from multipotent to unipotent) and differentiation capacity (from neurogenesis to gliogenesis). Recent development in tissue reprogramming allows for derivation of NSCs/NPCs from either healthy control subjects manipulated to carry disease mutations or affected individuals carrying specific disease-causing mutations allowing for detailed evaluation of cellular phenotype, pharmacological manipulation, and/or toxicological screening.In this chapter, we will discuss HPSC differentiation into neural stem cells (NSCs) and neurons. We will review the mechanism underlying in vivo neural differentiation and methods which recapitulate this in vitro. We describe a method of deriving NSCs and differentiated mature neurons highlighting key steps of the core protocol. We also provide detailed information of the transcription factor and morphogen map of the developing brain which can be used as a guide to derive region- and lineage-specific NSCs and differentiated neurons.