Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach.
The directed differentiation of pluripotent stem cells to a desired lineage often involves complex and lengthy protocols. In order to study the requirements for differentiation in a systematic way, we present here methodology for an iterative approach using combinations of small molecules and biological factors. The factors are used in a cyclical process in which the best combination …
