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Chemical Treatments for Insect Cell Differentiation: The Effects of 20-Hydroxyecdysone and Veratridine on Cultured Spodoptera frugiperda ( Sf 21) Insect Cell Ultrastructure

Previous studies have shown that insect cell cultures stop dividing, form clumps, and can be induced to grow processes reminiscent of axons, when the culture medium is supplemented with 20-hydroxyecdysone, insulin, or an agent that mimics their action, such as the ecdysone agonist, methoxyfenozide.Those cell growing processes resemble nerve cells, and the present study evaluates …

Chemical Treatments for Insect Cell Differentiation: The Effects of 20-Hydroxyecdysone and Veratridine on Cultured Spodoptera frugiperda ( Sf 21) Insect Cell Ultrastructure Read More »

Cyclic Stretch Promotes Proliferation and Contraction of Human Bladder Smooth Muscle Cells by Cajal-Mediated c-kit Expression in Interstitial Cells.

BACKGROUND The present study was performed to assess the effect of mechanical stretch on the proliferation and contractile function of hBSMCs. MATERIAL AND METHODS hBSMCs and ICCs were seeded at 8×104 cells/well in 6-well silicone elastomer-bottomed culture plates coated with type I collagen, and grown to 80% confluence in DMEM/10% FBS and a 5% CO₂ …

Cyclic Stretch Promotes Proliferation and Contraction of Human Bladder Smooth Muscle Cells by Cajal-Mediated c-kit Expression in Interstitial Cells. Read More »

PCR Controls

This section provides a comprehensive guide to PCR. It also includes guidelines and suggestions for maximizing results from your PCR. The photograph of one of Dr. Adlard’s polymerase chain reaction (PCR) experiment results compares amplified DNA of bivalve parasites Marteilia Sydney and its close relative Marteilia refringent. The same primers were used, with the DNA …

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Learning progression toward a measurement concept of fractions.

Learning progression toward a measurement concept of fractions.

Background Fractions proceed to pose a important problem for college kids and their lecturers alike. Mathematics training analysis signifies that the problem with fractions might stem from the constraints of part-whole ideas of fractions, which is the central focus of fractions curriculum and instruction within the USA. Students’ improvement of extra refined ideas of fractions, past the …

Learning progression toward a measurement concept of fractions. Read More »

Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach.

Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach.

The directed differentiation of pluripotent stem cells to a desired lineage often involves complex and lengthy protocols. In order to study the requirements for differentiation in a systematic way, we present here methodology for an iterative approach using combinations of small molecules and biological factors. The factors are used in a cyclical process in which the best combination …

Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach. Read More »

Generation of induced pluripotent stem cells from renal tubular cells of a patient with Alport syndrome.

Generation of induced pluripotent stem cells from renal tubular cells of a patient with Alport syndrome.

Alport syndrome (AS) is a hereditary disease that leads to kidney failure and is caused by mutations in the COL4A3, COL4A4, and COL4A5 genes that lead to the absence of collagen α3α4α5 (IV) networks in the mature kidney glomerular basement membrane. Approximately 80% of AS is X-linked because of mutations in COL4A5, the gene encoding …

Generation of induced pluripotent stem cells from renal tubular cells of a patient with Alport syndrome. Read More »

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